SYBR Green II RNA gel stain can be used to:
l Analyze small aliquots from RNA preparations before Northern blotting, start-site mapping or cDNA preparation l Visualize the migration behavior of 5S rRNA species after high-resolution denaturing gradient electrophoresis (DGGE) l Stain DNA in single-strand conformation polymorphism (SSCP) l Stain DNA before amplification by PCR
Materials Contents SYBR Green II stain is provided as a 10,000X concentrate in DMSO in the following sizes: l 50 µL (SY1040-50) l 500 µL (SY1040-500)
Storage Upon receipt, store the dye frozen at -20°C, protected from light in a desiccator. When stored properly, the SYBR Green II stain stock solution in DMSO is stable for six months to one year. 注：SYBR Green II主要用于RNA和单链DNA电泳。是EB和替代品，无致癌性，超敏感性。用作 常规电泳时，须将商品化的核酸MARKER作一定倍数的稀释（1/5-1/10），这样才能得到比较理想结果。
