CM9200-500mL L-15培养基
- 规格
产品类型：无菌过滤即用型液体培养基
渗透压：321±16 mOsm
酸碱度：7.2±0.2
储存：2-8℃储存，至少12个月。
Leibovitz's L-15Cell Culture Medium
IscoveL-15 (Leibovitz) 培养基最初开发用于非二氧化碳依赖型细胞生长环境（该环境需要碳酸氢钠）。
L-15 缓冲体系由盐类、碱性氨基酸、和替代glucose的替代物galactose维持。L-15支持HEp-2、LLC-MK2以及原代胚胎和成人组织的体外培养。多种病毒在此培养基中也成功得以包装。
产品特点：本品不含碳酸氢钠，支持细胞在无二氧化碳环境中生长，培养基缓冲体系由碱性氨基酸和半乳糖维持
产品类型：无菌过滤即用型液体培养基
渗透压：321±16 mOsm
酸碱度：7.2±0.2
储存条件：4-8ºC
包装规格：500mL
参考文献：Leibovitz, A. (1963). The Growth and Maintenance of Tissue/Cell Cultures in Free Gas Exchange with the Atmosphere. Amer. J. Hyg. 78:173-180.
产品组分：
保存条件： 2-8℃，避光保存，有效期 1 年
|
Components |
Molecular Weight |
Concentration (mg/L) |
mM |
|
Amino Acids |
|||
|
Glycine |
75.0 |
200.0 |
2.667 |
|
L-Alanine |
89.0 |
225.0 |
2.528 |
|
L-Arginine |
174.0 |
500.0 |
2.873 |
|
L-Asparagine |
132.0 |
250.0 |
1.893 |
|
L-Cysteine |
121.0 |
120.0 |
0.991 |
|
L-Glutamine |
146.0 |
300.0 |
2.054 |
|
L-Histidine |
155.0 |
250.0 |
1.612 |
|
L-Isoleucine |
131.0 |
250.0 |
1.908 |
|
L-Leucine |
131.0 |
125.0 |
0.954 |
|
L-Lysine |
146.0 |
75.0 |
0.513 |
|
L-Methionine |
149.0 |
75.0 |
0.503 |
|
L-Phenylalanine |
165.0 |
125.0 |
0.757 |
|
L-Serine |
105.0 |
200.0 |
1.904 |
|
L-Threonine |
119.0 |
300.0 |
2.521 |
|
L-Tryptophan |
204.0 |
20.0 |
0.098 |
|
L-Tyrosine |
181.0 |
300.0 |
1.657 |
|
L-Valine |
117.0 |
100.0 |
0.854 |
|
Vitamins |
|||
|
Choline chloride |
140.0 |
1.0 |
0.007 |
|
D-Calcium pantothenate |
477.0 |
1.0 |
0.002 |
|
Folic Acid |
441.0 |
1.0 |
0.002 |
|
Niacinamide |
122.0 |
1.0 |
0.008 |
|
Pyridoxine hydrochloride |
206.0 |
1.0 |
0.004 |
|
Riboflavin 5'-phosphate Na |
478.0 |
0.1 |
2.09E-4 |
|
Thiamine monophosphate |
442.0 |
1.0 |
0.002 |
|
i-Inositol |
180.0 |
2.0 |
0.011 |
|
Inorganic Salts |
|||
|
Calcium Chloride (CaCl2) (anhydrous) |
111.0 |
140.0 |
1.261 |
|
Magnesium Chloride (anhydrous) |
95.0 |
93.7 |
0.986 |
|
Magnesium Sulfate (MgSO4) (anhydrous) |
120.0 |
97.67 |
0.813 |
|
Potassium Chloride (KCl) |
75.0 |
400.0 |
5.333 |
|
Potassium Phosphate monobasic (KH2PO4) |
136.0 |
60.0 |
0.441 |
|
Sodium Chloride (NaCl) |
58.0 |
8000.0 |
137.931 |
|
Sodium Phosphate dibasic (Na2HPO4) anhydrous |
142.0 |
190.0 |
1.338 |
|
Other Components |
|||
|
D+ Galactose |
180.0 |
900.0 |
5.0 |
|
Phenol Red |
376.4 |
10.0 |
0.026 |
|
Sodium Pyruvate |
110.0 |
550.0 |
5.0 |
