描述产品
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes.
This MEM is modified as follows:
With |
Without |
• NEAA |
• HEPES |
• L-glutamine |
• Sodium Bicarbonate |
培养基配方
Components |
Molecular Weight |
Concentration (mg/L) |
mM |
Amino Acids |
Glycine |
75.0 |
7.5 |
0.1 |
L-Alanine |
89.0 |
8.9 |
0.099999994 |
L-Arginine hydrochloride |
211.0 |
126.0 |
0.5971564 |
L-Asparagine-H2O |
132.0 |
15.0 |
0.11363637 |
L-Aspartic acid |
133.0 |
13.3 |
0.1 |
L-Cystine 2HCl |
313.0 |
31.28 |
0.099936105 |
L-Glutamic Acid |
147.0 |
14.7 |
0.1 |
L-Glutamine |
146.0 |
292.0 |
2.0 |
L-Histidine hydrochloride-H2O |
210.0 |
42.0 |
0.2 |
L-Isoleucine |
131.0 |
52.0 |
0.39694658 |
L-Leucine |
131.0 |
52.0 |
0.39694658 |
L-Lysine hydrochloride |
183.0 |
72.5 |
0.39617488 |
L-Methionine |
149.0 |
15.0 |
0.10067114 |
L-Phenylalanine |
165.0 |
32.0 |
0.19393939 |
L-Proline |
115.0 |
11.5 |
0.1 |
L-Serine |
105.0 |
10.5 |
0.1 |
L-Threonine |
119.0 |
48.0 |
0.40336135 |
L-Tryptophan |
204.0 |
10.0 |
0.04901961 |
L-Tyrosine disodium salt dihydrate |
261.0 |
51.9 |
0.19885059 |
L-Valine |
117.0 |
46.0 |
0.3931624 |
Vitamins |
Choline chloride |
140.0 |
1.0 |
0.007142857 |
D-Calcium pantothenate |
477.0 |
1.0 |
0.002096436 |
Folic Acid |
441.0 |
1.0 |
0.0022675737 |
Niacinamide |
122.0 |
1.0 |
0.008196721 |
Pyridoxal hydrochloride |
204.0 |
1.0 |
0.004901961 |
Riboflavin |
376.0 |
0.1 |
2.6595744E-4 |
Thiamine hydrochloride |
337.0 |
1.0 |
0.002967359 |
i-Inositol |
180.0 |
2.0 |
0.011111111 |
Inorganic Salts |
Calcium Chloride (CaCl2) (anhyd.) |
111.0 |
200.0 |
1.8018018 |
Magnesium Sulfate (MgSO4) (anhyd.) |
120.0 |
97.67 |
0.8139166 |
Potassium Chloride (KCl) |
75.0 |
400.0 |
5.3333335 |
Sodium Chloride (NaCl) |
58.0 |
6800.0 |
117.24138 |
Sodium Phosphate monobasic (NaH2PO4-H2O) |
138.0 |
140.0 |
1.0144928 |
Other Components |
D-Glucose (Dextrose) |
180.0 |
1000.0 |
5.5555553 |
Phenol Red |
376.4 |
10.0 |
0.026567481 |